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Translational Control in Development and Disease Lab Institute of Medical Biology
 

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Translational Control in Development and Disease Lab
Institute of Medical Biology

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Achieving high transfection efficiency and cell viability at the same time has been a major challenge in gene transfer, especially involving difficult-to-transfect cells. Standard electroporator instruments which are not costly, often are ineffective and do not yield satisfactory efficiency and viability. Higher specification instruments come with a higher price tag and may require special reagents to guarantee satisfactory results.

Find out below how Insta bioAnalytik helped a customer achieve remarkable transfection efficiency without involving high investments.

"As a molecular biologist working with human embryonic stem cells and neuronal differentiation, transfection is a main tool to conduct my studies. When we were first looking for a transfection machine, we tried most brands of electroporators in the market and some liposome-mediated transfection methods. All turned out to be disappointing – with maximum efficiency only around 45-50%. Finally, we tested the NEPA21 with human embryonic stem cells, teratoma cell lines and primary keratinocytes. We obtained 85% transfection efficiency!" said Dr Nama. He also added that cell death could be an issue at times for some cell lines but it could be overcome by adjusting transfection parameters.

 The good thing is that we do not need to invest a lot in additional reagents. Just cuvettes & media work! This has since been the routine transfection method in our lab.- Dr Shrikant Nama, Senior Research Fellow
  Institute of Medical Biology