Department of Biochemistry
National University of Singapore
Developing the blot on an X-ray film is the conventional way of detecting protein signals. It involves handling and developing the film in a dark room. The technique of film developing is less popular recently; mainly due to short-linearity of data for quantification.
CCD imagers, on the other hand, converts the chemical signals to digital images. Once the membrane is taken out of the developing solution, it is placed directly on the imaging platform of the CCD imager. The subsequent procedures are automated – with the hit of the camera button, the imager will take multiple different exposures of the blot and save all images electronically. Accurate quantification is achieved by densitometry. When appropriate standards are used, molecular weight analysis is made possible.
CCD imagers are much more convenient and accurate. Syngene's new generation of gel documentation systems promises high quantum efficiencies and superior sensitivity. Users are able to effortlessly capture their chemiluminescence and multiplex images without much "guessing".