Achieving high transfection efficiency and cell viability at the same time has been a major challenge in gene transfer, especially involving difficult-to-transfect cells. Standard electroporator instruments which are not costly, often are ineffective and do not yield satisfactory efficiency and viability. Higher specification instruments come with a higher price tag and may require special reagents to guarantee satisfactory results.
Find out below how Insta bioAnalytik helped a customer achieve remarkable transfection efficiency without involving high investments.
"As a molecular biologist working with human embryonic stem cells and neuronal differentiation, transfection is a main tool to conduct my studies. When we were first looking for a transfection machine, we tried most brands of electroporators in the market and some liposome-mediated transfection methods. All turned out to be disappointing – with maximum efficiency only around 45-50%. Finally, we tested the NEPA21 with human embryonic stem cells, teratoma cell lines and primary keratinocytes. We obtained 85% transfection efficiency!" said Dr Nama. He also added that cell death could be an issue at times for some cell lines but it could be overcome by adjusting transfection parameters.
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Dr Shrikant Nama
“The good thing is that we do not need to invest a lot in additional reagents. Just cuvettes & media work! This has since been the routine transfection method in our lab.”